As an important step toward overcoming the technical and environmental limitations of current REE processing methods, the LLNL team has patented and demonstrated a biobased, all-aqueous REE extraction and separation scheme using the REE-selective lanmodulin protein. Lanmodulin can be fixed onto porous support materials using thiol-maleimide chemistry, which can enable tandem REE purification…

LLNL researchers have discovered that some inexpensive and commercially available molecules used for other applications, could render certain lanthanide and actinide elements highly fluorescent. These molecules are not sold for applications involving the detection of REEs and actinides via fluorescence. They are instead used as additives in cosmetic products and/or in the pharmaceutical…

LLNL’s high throughput method involves proteome-wide screening for linear B-cell epitopes using native proteomes isolated from a pathogen of interest and convalescent sera from immunized animals.

LLNL researchers have designed a synthetic, concatemeric bacterial expression vector that expresses a protein sequence that can be digested into a single peptide. The synthetic protein is designed to be secreted outside E. coli cells, and therefore can be purified using a His-tag from the cell supernatant (thereby reducing the need to lyse the cells for purification).

This invention consists of a functionalized membrane (e.g. polyethylene glycol (PEG)) and osmosis or electric potential as a driving force. The PEG membrane provides high biological particles separation and prevents sample for clogging due to the strong hydration of functional polymers layer and their resistance to protein adsorption.

This invention is an improved chromatography device that utilizes the concept of a functionally graded material (FGM) for separation of components. The technology consists of a device that contains a FGM that is patterned to have a gradient in material properties (e.g. chemical affinity, surface chemistry, chirality, pore size, etc.) normal to the direction of flow of the mobile phase. The…

Researchers at LLNL have developed a more efficient and cost-effective method and system for synthesizing a critical D-aminoluciferin precursor and related compounds. D-aminoluciferin is as active as luciferin and provides a free -NH2 group for functionalization to attach peptide sequences corresponding to the cleavage site of a protease. This allows for the synthesis of bioluminescent probes…

The described invention is a miniature fluidic device for separating particles suspended within a liquid sample that is introduced into the interior volume of the device. The device uses laminar flow and a combination of gravity and acoustic, electrophoretic, dielectrophoretic, and diffusion-based processes in concert to separate the different particle types and allow them to be collected…

Researchers at LLNL have developed a nanotube sensor (single-walled or multi-walled carbon nanotubes) enclosed within a highly selective lipid bilayer that can detect variations in ion transport using signal amplification generated from the disruption of protein pores across the lipid layer. Changes in the device’s transistor current are recorded by an external circuit with high efficiency as…

LLNL has a successful history of developing instruments for detecting and characterizing airborne pathogens. Often, aerosol characterizing instruments require highly focused particle beams with little or no transmission losses. In addition, they need to interface to the sampling environment with a very high sampling rate so that more aerosol particles can be collected and sensitivity can be…