The biotech industry aims to move towards an on-chip system for sample generation, amplification and detection of both DNA and RNA based organisms. LLNL has invented a new way of isolating samples in a system.
This invention enables creation of partitioned fluid "packets" between polymeric sheets for chemical separation, DNA amplification or PCR-based DNA detection. The polymeric films containing the fluid would be sealed upon application of heat and further partitioned into individual microliter or picoliter samples. This approach would allow a continuous flow of samples through the system and minimize reaction and processing times.
- Microfluidic systems can be used for sensitive assays and rapid detection of dilute analytes
- Fluid partitioning allows for multiple simultaneous analyses
- Isolation of samples will improve throughput and detection
- Continuous Flow and Processing of Samples
- Biological pathogen detection and identification
- Development of personalized medical therapies
- Biomedical applications, including single-cell analysis
- Food and beverage safety
LLNL has filed a patent application for this invention. LLNL seeks commercial partners to help develop this Microfluidic Partitioning system for PCR-based DNA detection.